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Product Description: SUMO2 is a Ubiquitin-like protein (UBL) that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. This SUMO2 substrate is C-terminally derivatized with a bis-Gly-Rhodamine 110 fluorophore. The bis-Gly-Rh110 is quenched until the amide bond between the C-terminal glycine and the rhodamine compound is hydrolyzed. The efficiency of quenching combined with the powerful signal upon hydrolysis yields an unparalleled signal-to-background. SUMO2-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 and SENP2, among other deSUMOylating enzymes. The substrate activity of SUMO2-Rhodamine 110 was determined by measuring the SENP1 catalyzed release of unquenched Gly-Rh-110.
Alternate Names/Synonyms: Small Ubiquitin-related Modifier 2; HSMT3; SMT3 Homolog 2; Sentrin-2; Ubiquitin-like Protein SMT3A
Product type: Protein
Source / Host: E. coli
Sequence: Human SUMO2 (aa1-93) conjugated at the C-terminus to a quenched Rhodamine 110 dye.
Species Crossreactivity: Human
Molecular Weight: ~11kDa
Labels - Conjugates: Rhodamine
Purity: >97% (LCMS)
Concentration: Lot dependent.
Formulation: Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride.
Handling Advice: Aliquot to avoid freeze/thaw cycles. Protect from light.
Short Term Storage: -80°C
Long Term Storage: -80°C
Shipping: DRY ICE
Use & Stability: Stable for at least 1 year after receipt when stored at -80°C.
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