SuperTrap™ Protein G Separopore® (Microbeads)

Applications:

• Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids.
• These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments.
• bioWORLD's SuperTrap™ Protein G Separopore affinity matrix is prepared with immobilized recombinant protein G (from Streptococcus sp.) by CNBr activation coupling method.
• Protein G Separopore Microbeads are used for purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.

Technical Specifications

• Ligand: rProtein-G (E. coli)
• Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)
• Matrix activation: Cyanogen bromide
• Particle size range: 30-50 µm
• Exclusion limit range: 6 x 10⁴ - 2 x 10⁷
• Ligand density ~ 4 mg Protein-G / ml drained medium
• Binding capacity: ~ 20 mg human IgG / ml drained gel
• pH stability: 3 - 9 (for use), 2 - 10 (for cleaning)
• Storage: 2 - 8 C
• Supplied as suspension in 0.5M NaCl