Triticum vulgaris Lectin (WGA) - BlackPearl®

BlackPearl-L (Wheat Germ Lectin)

Wheat germ lectin (Isolated from Triticum vulgaris) conjugated to BlackPearl Magnetic Beads.

Wheat germ agglutinin is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin has proven useful for the purification of insulin receptors and for neuronal tracing.

Technical Specifications:

Ligand Source: Triticum vulgaris

Matrix: BlackPearl™ 4B (agarose, 4%) w/ paramagnetic core

Particle size range: 52 - 165 µm

Matrix activation: Cyanogen bromide

Matrix attachment: Amino

Ligand density: 2 mg Wheat Germ / mL of settled gel

Binding Capacity: 2 mg/mL

Inhibiting/Eluting Sugar: Chitin hydrolysate or GlcNAc with acid or salt

Carbohydrate for Elution: 500mM N-acetylglucosamine, Chitin Hydrolysate or 100 mM acetic acid

Blood Group Specificity: A, B, O

Form: 50:50 Liquid Suspension, PBS (1x) pH 7.4 w/ 0.01% Thimerosal

pH stability: 4 - 9

Storage: 2 - 8 C, DO NOT FREEZE

Application note: Calcium is required for binding.

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.