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Wheat Germ Agglutinin (WGA)-Coupled Polystyrene Beads
Wheat germ lectin isolated from Triticum vulgaris is a 36 kDa protein/glycoprotein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues. This lectin has proven useful for the purification of insulin receptors and for neuronal tracing studies. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Other applications include blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes and fractionation of cells and other particles.
bioWorldâ€s Wheat Germ Lectin (WGA) coupled polystyrene beads are supplied as a 5% suspension.
Technical Specifications:
Ligand Source: Triticum vulgaris
Matrix: Polystyrene Beads
Matrix activation: EDC
Matrix attachment: Amide
Ligand density: 1-2 mg Wheat Germ / mL of settled gel
Binding Capacity: 2-3 mg/mL
Inhibiting/Eluting Sugar: Chitin hydrolysate or GlcNAc with acid or salt
Carbohydrate for Elution: 0.2M Chitin hydrolysate or GlcNAc with acid or salt
Blood Group Specificity: A, B, O
Form: 50:50 Liquid Suspension, PBS (1x) pH 7.4 w/ 0.01% Thimerosal
pH stability: 4 - 9
Storage: 2 - 8 C, DO NOT FREEZE
Application note: Calcium is required for binding.
References
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