Triticum vulgaris Lectin (WGA) - Separopore® 4B

Applications:

Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). WGA-Separopore 4B-CL has proven useful for the purification of insulin receptors and for neuronal tracing studies. WGA-Separopore 4B-CL is also used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Agarose bound Wheat Germ Agglutinin is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2 x 10⁷ are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions.

Technical Specifications:

• Ligand Source: Triticum vulgaris
• Matrix: Separopore 4B-CL (crosslinked agarose, 4%)
• Particle size range: 52 - 165 µm
• Matrix activation: Cyanogen bromide

• Matrix attachment: Amino

• Ligand density: 5-10 mg Wheat / mL drained gel
• Binding capacity: 8 mg of N-acetylglucosaminyl glycoprotein/ml drained gel
• Inhibiting/Eluting Sugar: Chitin hydrolysate or GlcNAc with acid or salt
• Carbohydrate for Elution: 500 mM N-acetylglucosamine or 100 mM acetic acid

• Blood Group Specificity: A, B, O

• Form: 50:50 Liquid Suspension, PBS (1x) pH 7.4 w/ 0.01% Thimerosal

• pH stability: 4 - 9
• Storage temp: 2 - 8 C, DO NOT FREEZE
• Application note: Calcium is required for binding.

References:

• Presence of a large Beta(1-3)glucan linked to chitin at the Saccharomyces cerevisiae mother-bud neck suggests involvement in localized growth control. Eukaryot Cell. (2012) 11: 388-400.
• Localization and function of a eukaryotic-initiation-factor-2-associated 67-kDa glycoprotein. World J Biol Chem. (2010) 1: 313-20.
• Purification, immunochemical quantification and localization in rat heart of putative fatty acid translocase (FAT/CD36). Mol Cell Biochem. (2006) 284: 127-34.
• Nuclear localization of STAT5A modified with O-linked N-acetylglucosamine and early involution in the mammary gland of Hirosaki hairless rat. J Biol Chem. (2005) 280: 43010-6.
• Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1). Cell Res. (2001) 11: 28-36.
• Purification and characterization of fetal bovine serum beta-N-acetyl-D-galactosaminyltransferase and beta-D-glucuronyltransferase involved in chondroitin sulfate biosynthesis. Eur J Biochem. (1999) 264: 461-7.
• Identification of the cyclosporin-binding site in P-glycoprotein. Biochemistry. (1998) 37: 18110-8.
• Purification, cDNA cloning, and expression of UDP-Gal: glucosylceramide beta-1,4-galactosyltransferase from rat brain. J Biol Chem. (1998) 273: 13570-7.
• ATPase activity of purified multidrug resistance-associated protein. J Biol Chem. (1997) 272: 30962-8.
• Characterization of insulin and insulin-like growth factor-I ovarian receptors during the reproductive cycle of carp (Cyprinus carpio). Biol Reprod. (1997) 56: 1126-32.
• Dystrophin-glycoprotein complex purified from hamster cardiac muscle. Comparison of the complexes from cardiac and skeletal muscles of hamster and rabbit. J Mol Cell Cardiol. (1996) 28: 2501-9.
• Purification and characterization of an insulin-stimulated insulin receptor serine kinase. Biochemistry. (1996) 35: 14340-51.
• Expression and characterization of gastrin-releasing peptide receptor in normal and cancerous pancreas. Pancreas. (1996) 12: 25-35.
• Purification, photoaffinity labeling, and characterization of a single enzyme for 6-sulfation of both chondroitin sulphate and keratan sulfate. J Biol Chem. (1995) 270: 22483-7.
• The major integral membrane glycoprotein in adipocytes is a novel 200-kDa heterodimer. Mol Membr Biol. (1995) 12: 277-81.
• Abundant insulin-like growth factor-1 (IGF-1) receptor binding in fish skeletal muscle. Gen Comp Endocrinol. (1995) 98: 16-25.
• Insulin binding and receptor tyrosine kinase activity in skeletal muscle of carnivorous and omnivorous fish. Am J Physiol. (1994) 266: R1944-50.
• A candidate molecule for the matrix assembly receptor to the N-terminal 29-kDa fragment of fibronectin in chick myoblasts. J Biol Chem. (1994) 269: 7651-7.
• Identification and isolation of glycoproteins that translocate to the cell surface from GLUT4-enriched vesicles in an insulin-dependent fashion. J Biol Chem. (1994) 269: 138-42.
• Purification of chondroitin 6-sulfotransferase secreted from cultured chick embryo chondrocytes. J Biol Chem. (1993) 268: 21968-74.

Related products:

• Ulex europaeus agglutinin (UEA) Separopore 4B-CL (SKU # 20120004)
• Lens culinaris (Lentil) Agglutinin-Separopore 4B-CL (SKU # 20120003)
• Glycine max (soybean) Lectin-Separopore 4B-CL (SKU # 20120002)
• Erythrina cristagalli (coral tree) Lectin-Separopore 4B-CL (SKU # 20120006)
• Concanavalin A (Con-A) Separopore 4B-CL (SKU # 20120005)
• Jacalin (Artocarpus integrifolia) Separopore 4B-CL (SKU # 20120001)
• Arachis hypogaea (Peanut) Agglutinin-Separopore 4B-CL (SKU # 20120000)

Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.