Triticum vulgaris Lectin (WGA) - Separopore® 6B

Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). WGA-Separopore 6B-CL has proven useful for the purification of insulin receptors and for neuronal tracing studies. WGA-Separopore 6B-CL is also used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Agarose bound Wheat Germ Agglutinin is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2 x 10⁷ are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions.

Technical Specifications:

Ligand Source: Triticum vulgaris

Matrix: Separopore 4B-CL (crosslinked agarose beads, 4%)

Particle size range: 52 - 165 µm

Matrix activation: Cyanogen bromide

Matrix attachment: Amino

Ligand density: 2 mg Wheat / mL of settled gel

Binding Capacity: 2-3 mg/mL

Inhibiting/Eluting Sugar: Chitin hydrolysate or GlcNAc with or salt

Carbohydrate for Elution: 0.2M Chitin hydrolysate or GlcNAc with or salt

Blood Group Specificity: A, B, O

Form: 50:50 Liquid Suspension, PBS (1x) pH 7.4 w/ 0.01% Thimerosal

pH stability: 4 - 9

Storage: 2 - 8 C, DO NOT FREEZE

Application note: Calcium is required for binding.