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AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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Product description: Protein. Human UBE2D3 (aa1-147)is untagged. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 8, 200mM sodium chloride, 10% glycerol (v/v), 1mM TCEP. Purity: >95% (SDS-PAGE). UBE2D3 (UbcH5c) is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D3 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D3-Ub thioester. UBE2D3 is capable of associating with numerous known E3 ligases including MDM2, which target proteins such as p53 for proteasomal degradation through polyubiquitination.
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