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Collagenase NB 4 Standard Grade Nr: S1745402
500 mg
photos non contractuelles
Détails Produit
Nordmark Collagenase NB - first choice for high yield and viable cells
As Collagenase NB 4 Standard Grade has comparable enzymatic properties to Collagenase NB 5 Sterile Grade (Cat. no. N0002778) and Collagenase NB 6 GMP Grade (Cat. no.N0002779), this enzyme might also be used in order to establish applications which have to meet sterile or GMP requirements like tissue engineering and transplantation.
Specifications:
Contains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities.
PZ activity (Wünsch): ≥ 0.10 U/mg
Suitable for different applications in tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]
Order Information
| Product name | Cat.No | Size | EUR |
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Collagenase NB 4 Standard Grade |
Nordmark : S1745402 Serva : 1745402 |
500 mg | |
| Collagenase NB 4 Standard Grade |
Nordmark : S1745401 Serva : 1745401 |
1 g |
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Collagenase NB 4 Standard Grade |
Nordmark : S1745403 Serva : 1745403 |
5 g |
Storage Temperature: +2 °C to +8 °C
Related Products
Collagenase NB 4 Standard Grade is often used to establish applications and to optimize protocols. If later on, the application meets sterile or GMP requirements, Collagenase NB 5 Sterile Grade (Cat. No. N0002778) or Collagenase NB 6 GMP Grade (Cat. No. N0002779) can be used. Both products have comparable enzymatic properties to Collagenase NB 4 Standard Grade.
References and Definitions
Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
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