anti-Apaf-1, mAb (18H2)

Product description: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Alternate Names/Synonyms: Apoptosis Protease-activating Factor 1

Product Type: Monoclonal Antibody

Clone: 18H2

Isotype: Rat IgG2akappa

Immunogen: Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.

Applications: ELISA, ICC, IP, WB

Species Crossreactivity: Human, Mouse

Specificity: Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1.

Endotoxin: <0.1EU/µg purified protein (LAL test; Lonza).

Concentration: 1mg/ml

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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