anti-Cardif (human), mAb (Adri-1)

Product description: RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.

Alternate Names/Synonyms: CARD Adapter Inducing Interferon-beta; IPS-1; MAVS; Mitochondrial Antiviral Signaling Protein; VISA; Virus-induced Signaling Adapter; Interferon-beta Promoter Stimulator Protein 1

Product Type: Monoclonal Antibody

Clone: Adri-1

Isotype: Mouse IgG2b

Immunogen: Recombinant human Cardif (aa 160-450).

Applications: ICC, IHC, IP, WB

Species Crossreactivity: Human

Specificity: Recognizes human Cardif.

Endotoxin: <0.001EU/µg purified protein or <1EU/mg purified protein (LAL test; Lonza).

Concentration: 1mg/ml

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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