anti-NF-kappa-B p65 (human), Rabbit Monoclonal (RM273)

Product description: NF-kappaB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappaB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at kappaB sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappaB sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappaB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappaB inhibitor (I-kappaB) family. In a conventional activation pathway, I-kappaB is phosphorylated by I-kappaB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappaB complex which translocates to the nucleus. NF-kappaB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappaB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappaB complex.

Alternate Names/Synonyms: RELA; NFKB3; Transcription Factor p65; Nuclear Factor NF-kappaB p65 Subunit; Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 3

Product Type: Recombinant Antibody

Clone: RM273

Isotype: Rabbit IgG

Immunogen: A peptide corresponding to the area near the C-terminus of human transcription factor p65 (NFkB p65 subunit).

Applications: IHC, WB

Species Crossreactivity: Human

Specificity: This antibody reacts to human transcription factor p65 (NF-kB p65 subunit).

Concentration: N/A

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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