anti-NLRC3 (mouse), mAb (Eowyn-1)

Product description: The novel nucleotide oligomerization domain (NOD)-like receptor (NLR) with a caspase activation and recruitment domain (CARD) 3 (NLRC3) protein belongs to the NLR family of cytosolic pathogen recognition receptors. NLRC3 has the characteristic NOD and leucine-rich repeat configuration with a less well defined CARD. NLRC3 is expressed in T lymphocytes and may be involved in suppression of T cell activation. NLRC3 is a cytoplasmic protein that negatively regulates pro-IL-1beta maturation and inhibits toll-like receptor (TLR)-dependent activation of the transcription factor NF-kappaB. NLRC3 interacts with the TLR signaling adaptor TRAF6 to attenuate Lys63 (K63)-linked ubiquitination of TRAF6 and activation of NF-kappaB. NLRC3 also reduces STING-dependent innate immune activation in response to cytosolic DNA, cyclic di-GMP (c-di-GMP) and DNA viruses. NLRC3 associated with both STING and TBK1, impedes STING-TBK1 interaction and downstream type I interferon production.

Alternate Names/Synonyms: NOD3; Nucleotide-binding Oligomerization Protein 3; CARD15-like Protein; Caterpiller Protein 16.2; CLR16.2

Product Type: Monoclonal Antibody

Clone: Eowyn-1

Isotype: Mouse IgG1

Immunogen: Recombinant mouse NLRC3 (card domain).

Applications: WB

Species Crossreactivity: Mouse

Specificity: Detects overexpressed mouse NLRC3. Doest not detect human NLRC3. Although NLRC3 (mouse), mAb (Eowyn-1) is strong and specific (see figure), the endogenous protein has not been detected yet with this antibody (using NLRC3 KO versus WT immune cells). NLRC3 protein is probably expressed at very low levels or its translation / stability is triggered by stimuli still to be discovered .

Endotoxin: <0.01EU/µg purified protein (LAL test; Lonza).

Concentration: 1mg/ml

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

Lien vers la Fiche Technique