anti-PARP-10 [ARTD10] (human), mAb (5H11)

Product description: PARP-10 [ARTD10] is a mono-ADP-ribosyltransferase and was initially identified as an interaction partner of the proto-oncoprotein Myc. PARP-10 possesses ubiquitin-interaction motifs, which are important to inhibit the activation of NF-kappaB and downstream target genes in response to interleukin-1beta [IL-1beta] and tumor necrosis factor-alpha [TNF-alpha]. PARP-10 mono(ADP-ribosyl)ates (MARylates) NEMO (NF-kappaB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono(ADP-ribosyl)ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.

Alternate Names/Synonyms: ADP-ribosyltransferase Diphtheria Toxin-like 10; Poly(ADP-ribose) Polymerase-10

Product Type: Monoclonal Antibody

Clone: 5H11

Isotype: Rat IgG1

Immunogen: Recombinant human PARP-10 [ARTD10] (aa 1-907) fused to a GST-tag.

Applications: ICC, IP, WB

Species Crossreactivity: Human

Specificity: Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region.

Short Term Storage: +4°C

Long Term Storage: +4°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at +4°C.

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