anti-Survival Motor Neuron Protein, mAb (7B10)

Product description: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs. It is expressed in a wide variety of tissues, including high levels in brain, kidney and liver, moderate levels in skeletal and cardiac muscle and low levels in fibroblasts and lymphocytes. Mutations in SMN cause spinal muscular atrophy 1 (SMA1). Spinal muscular atrophy is a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. The antibody was used extensively to purify and characterize the macromolecular SMN complex from cultured human cells. The antibody binds with high affinity and specificity to its epitope, which comprises a sequence of up to 9 amino acids in the N-terminal part of human SMN. It allows the native elution of bound antigen with an excess of the cognate peptide. Via addition of the 7B10 epitope (TagIt epitope) to other proteins this purification strategy can also be exploited for purification of these fusion proteins and RNA-protein complexes from cells lacking this epitope on endogenous proteins, such as cells derived from C. elegans and Drosophila.

Alternate Names/Synonyms: SMN; Gemin-1; Component of Gems 1; BCD541

Product Type: Monoclonal Antibody

Clone: 7B10

Isotype: Mouse IgG1

Immunogen: Recombinant human full-length survival motor neuron protein (SMN).

Applications: IP, WB

Species Crossreactivity: Human

Specificity: Recognizes a sequence of up to 9 amino acids in the N-terminal domain of human SMN.

Concentration: 2mg/ml

Short Term Storage: +4°C

Long Term Storage: -20°C

Shipping: BLUE ICE

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C.

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