Fc (silent) InVivoKine™ Human IgG1 Control

Product Description: Negative control in experiments with recombinant proteins fused to human IgG1 Fc (LALA-PG)-KIH (Knobs-into-Holes) (e.g. Prod. No. AG-40B-0248). This protein is not an appropriate negative control for other standard Fc protein constructs. The Fc (silent) InVivoKine™ (human) IgG1 Control is produced by using two different vectors, one encoding for the Fc (LALA-PG) Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc (silent) InVivoKine™ (human) IgG1 Control. The LALA-PG mutations inhibit binding to Fc?Rs and C1q while FcRn binding and Fc stability remain unaffected. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

Alternate Names/Synonyms: Fc (LALA-PG) Knobs-into-Holes (human) IgG1; Immunoglobulin 1 Fragment Constant

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