ITCH E3 Ligase TR-FRET Kit

Product description: South Bay Bio’s ITCH TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto ITCH in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto ITCH in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.

Alternate Names/Synonyms: AIF4; AIP4; NAPP1

Specificity: Readout:Endpoint/KineticLabel or Dye:Europium-Cryptate & Cy5Detection Method:TR-FRETSubstrate Properties: Protein-based Substrate. Typical experimental concentration is 1x.Excitation/Emission (nm): 304/620 & 304/665

Crossreactivity: Human

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