Mdm2 E3 Ligase TR-FRET Kit

Product description: South Bay Bio’s MDM2 TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto GST-MDM2 in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto MDM2 in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.

Alternate Names/Synonyms: HDM2

Specificity: Readout:Endpoint/KineticLabel or Dye:Europium-Cryptate & Cy5Detection Method:TR-FRETSubstrate Properties: Protein-based Substrate. Typical experimental concentration is 1x.Excitation/Emission (nm): 304/620 & 304/665

Crossreactivity: Human

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