Parkin E3 Ligase TR-FRET Kit

Product description: South Bay Bio’s Parkin TR-FRET Ubiquitin Kit provides a fast and sensitive method monitoring ubiquitin conjugation onto both wild-type Parkin and a more active previously phosphorylated Parkin in solution, resulting from an enzymatic ubiquitin cascade without the need of running and staining an SDS gel. The kit enables continuous TR-FRET detection of ubiquitin chain formation onto Parkin in a real-time detection setup, or in an end-point configuration if desired. TR-FRET uses the extended fluorescence emission decay lifetimes typical of rare-earth lanthanides to impart a short time-delay between FRET donor excitation and emission. This delay provides a means to separate “true” signal from short-lived background fluorescence, and reduce interference from compound fluorescence and other assay artifacts.

Alternate Names/Synonyms: E3 Ubiquitin-protein Ligase Parkin; PARK2; Parkinson Juvenile Disease Protein 2; AR-JP; E3 Ubiquitin Ligase; LPRS2; Parkinson Disease Protein 2; PDJ; PRKN

Specificity: Readout: Endpoint/Kinetic Label or Dye: Europium-Cryptate & Cy5 Detection Method: TR-FRET Substrate Properties: Protein-based Substrate. Typical experimental concentration is 1x. Excitation/Emission (nm): 304/620 & 304/665

Crossreactivity: Human

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