Collagenase NB 1 GMP Grade Nr: N0002937

Contains chromatographically highly purified class I and class II collagenase (1).
Largely free from clostripain, trypsin-like protease and neutral protease.
Vial contains not less than 2000 PZ U collagenase.
PZ activity (Wünsch): ≥ 3.000 U/mg
Endotoxin ≤ 10.0 EU/mg (Ph. Eur.)
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.).
Manufactured according to EU GMP guidelines.
(Clostridiopeptidase A)
EC 3.4.24.3 • M_r ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]

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Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.

Collagenase NB 1 GMP Grade is often used in combination with Neutral Protease GMP Grade (Cat. No. N0002936). Nordmark also offers Collagenase AF-1 GMP Grade (Cat. No. N0003554) and Neutral Protease AF GMP Grade (Cat. No. N0003553) which are completely free of any animal based components excluding the risk of transmission of potential animal-derived pathogens. For research purpose, Nordmark also provides research grade alternatives like Collagenase NB 1 Premium Grade (Cat. No. S1745503) and Neutral Protease NB (Cat. No. S3030112).

*Unit definitions: Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
**Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)].

References:
1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51

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