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AVANTI POLAR LIPIDS , CYGNUS , LARODAN , ADIPOGEN , MYBIOSOURCE , SERVA NORDMARK, BIOWORLD, EDGEBIO , PANREAC APPLICHEM
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Metalloprotease suitable for tissue dissociation applications. For cell isolation Neutral Protease NB GMP Grade is used in combination with purified Collagenase NB 1 GMP Grade (Cat. no. N0002937) or Collagenase NB 6 GMP Grade (Cat. no. N0002779) for applications which have to meet GMP requirements like tissue engineering and transplantation into humans.
Contains chromatographically purified neutral protease.
Vial contains not less than 100 DMC U of neutral protease.
Neutral protease activity (DMC): ≥ 0.50 U/mg
Endotoxin: ≤ 100 EU/mg (Ph. Eur.)
Largely free of collagenolytic activity.
Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia, abnormal toxicity (general safety test; Ph. Eur.).
Manufactured according to EU GMP guidelines.
Mr ca. 34 000 • CAS [9001-92-7]
Product name | Cat.No | Size | EUR |
Neutral Protease NB GMP Grade |
Nordmark : N0002936 Serva : 3030301 |
≥ 100 DMC U |
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Storage Temperature: +2 °C to +8 °C
This article will be delivered cooled on blue ice.
For cell isolation, Neutral Protease NB GMP Grade is often used in combination with Collagenase NB 1 GMP Grade (Cat. No. N0002937). Nordmark also offers animal-free products, Neutral Protease AF GMP Grade (Cat. No N0003553) and Collagenase AF-1 GMP Grade (Cat. No. N0003554) which are completely free of any animal based components excluding the risk of transmission of potential animal-derived pathogens. For research purpose, Nordmark provides research grade alternatives like Neutral Protease NB (Cat. No. S3030112) and Collagenase NB 1 Premium Grade (Cat. No. S1745503).
Unit definition:
Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).
Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).
References:
1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93.
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